ABSTRACT
OBJECTIVE@#To determine the effects of long-term estrogen replacement treatment on blood pressure and expressions of insulin receptor (IR) and insulin receptor substrate-1 ( IRS-1) in myocardium.@*METHODS@#Fifty female SD rats were randomly divided into 3 groups. And then sham ( n = 16), ovariectomy (OVX, n = 17), and estrogen replacement treatment group (OVX + E2, n = 17) were established. Systolic blood pressure of tail artery was determined by tail-cuff technique before the operation and on week 12 after the operation. The expressions of IR and IRS-1 were measured by RT-PCR in myocardium of SD rats.@*RESULTS@#Blood pressure [ (118.75+/-2.77) mmHg] in OVX was significantly higher than that in the sham [ ( 103.86+/-1.84) mmHg, P 0.05 ). The difference of IR expression has no statistical significance among the 3 groups.@*CONCLUSION@#Long-term estrogen replacement treatment might protect cardiovascular system through decreasing the blood pressure and inducing the expression of IRS-1 in myocardium. However, plasma estrogen level doesn't significantly influence the IR expression.
Subject(s)
Animals , Female , Rats , Blood Pressure , Estradiol , Pharmacology , Estrogen Replacement Therapy , Insulin Receptor Substrate Proteins , Myocardium , Metabolism , Ovariectomy , Phosphoproteins , Genetics , Rats, Sprague-Dawley , Receptor, Insulin , Genetics , Time FactorsABSTRACT
<p><b>OBJECTIVE</b>To investigate the frequencies distribution of GSTT1 and GSTM1 null genotypes in 24 Yunnan populations.</p><p><b>METHODS</b>GSTT1 and GSTM1 genotypes were analyzed by PCR procedure.</p><p><b>RESULTS</b>The range of frequencies for GSTT1 and GSTM1 null genotypes in the populations were 0.188-0.633 and 0.400-0.745, respectively, and in the districts were 0.286-0.583, 0.433-0.745 respectively. There was significant relationship between GSTT1 frequencies and populations.</p><p><b>CONCLUSION</b>The frequencies of GSTT1 and GSTM1 null genotypes in 24 Yunnan populations were different, but they were almost the same in different districts of Yunnan.</p>
Subject(s)
Humans , China , DNA , Genetics , Gene Frequency , Genotype , Glutathione Transferase , Genetics , Polymerase Chain ReactionABSTRACT
<p><b>OBJECTIVE</b>To study the frequency distribution of flavin-containing monooxygenase 3 (FMO3) mutant alleles in 28 populations originating from 24 ethnic minorities in Yunnan of China.</p><p><b>METHODS</b>FMO3 genotypes were analyzed by polymerase chain reaction-restriction fragment length polymorphism.</p><p><b>RESULTS</b>The average frequencies of FMO3/Stop(148), FMO3/Lys(158) and FMO3/Gly(308) were 0.395(0.174-0.803), 0.208 (0.056-0.414), 0.046(0-0.217), respectively. The frequencies of FMO3/Gly(308) in Blang, Huayaodai, Shuidai, Zhuang, De'ang, Jingpo, Nu and Hui populations were null.</p><p><b>CONCLUSION</b>It was found that the frequencies of FMO3 mutant alleles varied not only in different ethnic groups, but also in different populations that stemmed from the same ethnic group.</p>
Subject(s)
Humans , Alleles , China , Gene Frequency , Genotype , Mutation , Oxygenases , GeneticsABSTRACT
Objective To understand the changes and possible mechanism of the blood brain barrier(BBB) permeability induced by tumor necrosis factor(TNF-?) in vitro.Methods BBB model was established by coculturing allogenic brain microvessel end othelial cell(BMEC) and astrocyte(AS).The BBB model was divided randomly into normal control group,TNF-? group and Y-27632 pretreatment group.The changes of BBB permeability were evaluated by Gamma radioim muno assay counter.Results The Gamma radioimmuno assay indicated that the marker,~(125)I-BSA,across the BBB model in vitro was significantly increased after TNF-? treatment compared with control group,Y-27632 pretreatmen could prevent the permeability of BBB induced by TNF-?(P